Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients

Date: 7-29-2010

(2009)Paul Cohen-Bacrie, PC, Belloc S, Menezo, Y, Clement, P, Jamal H, Benkhalifa, M.Laboratoire d'Eylau, Paris, France bLaboratoire Clement, Le Blanc Mesnil, France Fertility and Sterility 91:1801-05 Objective: To investigate DNA fragmentation by using terminal deoxyribonucleotidyl transferase–mediated dUTP nick-end labeling in relation to World Health Organization parameters and computer-aided sperm analysis (CASA) in sperm to determine the possibility of obtaining a correlation among CASA parameters, sperm morphology, and DNA fragmentation. Design: Sperm analysis according to World Health Organization parameters, terminal deoxyribonucleotidyl transferase–mediated dUTP nick-end labeling (TUNEL) for sperm DNA fragmentation, and CASA for sperm movement. Prospective study. Patient(s): One thousand six hundred thirty-three men who were referred for infertility investigation, including a complete sperm analysis. Result(s): One third of the patients had a TUNEL rate of >30%. Analysis of the 21 semen parameters tested revealed that 7 of them were significantly correlated with the TUNEL results. Conclusion(s): World Health Organization sperm parameters and DNA damage are complementary, rather than strongly linked. This should be considered to more fully understand the paternal contribution in assisted reproductive technologies failures. DPE: Note that nearly 1/3 of patients in this study had a high % DFI as measured by Tunel assay—the results may be similar to that found by SCSA. In any case, as high percent of couple sub/infertility have the DNA fragmentation defect that the SCSA can detect. Action point: Consider doing an SCSA test early in your TTC efforts to determine if sperm DNA fragmentation is the factor that is limited good pregnancy success. ---------------------------------------------------------------------------------------------------