Sperm chromatin structure is associated with the quality of spermatogenesis in infertile patients

Date: 7-29-2010

(2010), Fertility and Sterility 75:14-19. Smit M, Romin JC, Wildhagen MF, Weber FA, Dohle G. Andrology Unit of the Department of Urology, Erasmus MC Rotterdam, Rotterdam, The Netherlands Objective: To establish the diagnostic value of sperm chromatin structure assessment for the evaluation of male factor infertility, in addition to conventional andrological workup. Patient(s): Two hundred seventy-nine male partners of infertile couples. Main Outcome Measure(s):The DNA fragmentation index (DFI) determined by the sperm chromatin structure assay (SCSA), semen parameters, serum levels of reproductive hormones, and World Health Organization (WHO) classification of male factor sub fertility. Result(s): In all patient categories, except those including patients with hypogonadotrophic hypogonadism, sperm antibodies, or normospermia, DFI was significantly higher compared with in proven fertile controls. After classification of the quality of spermatogenesis based on mean testicular volume (<10 ml vs. >15 ml), follicle stimulating hormone (FSH; > 10 U/L vs. <5 U/L), and inhibin-B (<100 nmol/L vs. >150 nmol/L), the DFI was significantly higher in patients with poor spermatogenesis (35.9%) than in patients with normal spermatogenesis (25.9%). In a multiple regression analysis, the teratozoospermia index, sperm vitality, and FSH were significant determinants of the DFI level. Male age was associated with DFI, but leukocytospermia, body mass index, and smoking were not confounders of DFI. Conclusion(s): Impaired spermatogenesis, irrespective of the WHO classification of male DPE: Note again that while %DFI is higher in patients with poor spermatogenesis, patients with NORMAL spermatogenesis could also have a high %DFI that is at a level that may cause sub/infertility. Also, TIME WAITS FOR NOT MAN. The older the man the less likely for having a timely pregnancy. (click here for AGE paper).